Coding
Part:BBa_K3382000:Design
Designed by: Leung Chung Ki Group: iGEM20_HK_CPU-WFN-WYY (2020-10-14)
H. insolens cutinase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We optimized the codons to make it suitable to be expressed in E. coli.
Source
https://www.sciencedirect.com/science/article/abs/pii/S0021967305005947 https://link.springer.com/article/10.1007/s00253-019-09717-y https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505623/#CR99 https://pubs.acs.org/doi/pdf/10.1021/ma9005318 https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.12714 https://pubs.acs.org/doi/abs/10.1021/jp065896u